Tetraspanins comprise a large superfamily of cell surface-associated membrane proteins with four transmembrane domains. On cell membranes, tetraspanins form networks of various proteins called tetraspanin-enriched microdomains (TEMs). CD63 was the first characterized tetraspanin and it is found in TEMs, as well as late endosomes and lysosomes. In late endosomes, CD63 is enriched on the intraluminal vesicles, and can be secreted as exosomes through fusion of endosomes with the plasma membrane. The complex localization pattern of CD63 suggests that its intracellular trafficking and distribution must be tightly regulated. CD63 contains N-link glycosylation sites that produce diverse CD63 molecules that range from 30 to 60 kDa depending on cell type. CD63 is an important exosomal marker in cancer cells, and may be involved in cancer progression and metastasis.
References
Pols, MS & Klumperman, J (2009) Exp Cell Res. 315(9):1584. Metzelaar, M.J. et al. (1991) J Biol Chem. 266(5):3239.Azorsa, D.O. et al. (1991) Blood. 78(2):280.
Western blot of native human cell lyastes: MeWo (lane 1), and MeWo treated with PNGase F (lane 2), and A549 (lane 3), and A549 treated with PNGase F (lane 4). The blot was probed with mouse monoclonal CD63 (CM0401) at 1:100.
Immunocytochemical labeling of CD63 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal CD63 (CM0401). The antibody was detected using goat anti-mouse Ig DyLight® 594.
*For more information, see UniProt Accession P08962
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
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KIT SUMMARY
ECM Biosciences是一家著名的生物公司，开发新的生物技术的生命科学研究。公司工程与研究机构全球发展创新的工具，以提高研究和治疗相关的神经病理学，癌症，免疫功能障碍和心血管疾病。ECM Biosciences 的科学合作重点在分子组成部分，产生细胞运动的变化，细胞形状，促进细胞运动和细胞结构确定。细胞运动的重要作用在肿瘤细胞迁移，免疫细胞浸润，以及神经和血管修复。各种蛋白质在细胞外机质（ECM）周围细胞信号蛋白在细胞内的细胞骨架，以控制细胞运动 ECM Biosciences 生物利用创新肽和抗体为基础的办法，发展新的工具来检测和操作蛋白质功能的各个方面的细胞运动，如细胞黏附，细胞迁移和细胞信号。
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