Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.
References
Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808. Marte, B. & Downward. J. (1997) TIBS. 22:355-358.Jones, P.F. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4171-4175.
Western blot analysis of A431 cells, serum starved overnight (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Akt (Thr-34) (lanes 1 & 2) or anti-Akt1 (N-terminal region) (lanes 3 & 4).
Western blot analysis of A431 cells untreated (lanes 1 & 3) or treated with 100 ng/ml EGF for 60 min. (lanes 2 & 4). The blots were probed with monoclonal anti-phospho-Akt (Ser-473) (lanes 1 & 2) and monoclonal anti-Akt1 (N-terminal region) (lanes 3 &4).
*For more information, see UniProt Accession P31749
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
Product References:
Wei, H. & Vander Heide, R. (2010) AJP Heart Circ. 298:H152. (WB: rat myocardium)Li, W. et al. (2009) Am J Physiol Cell Physiol (2009) 297:706. (WB: mouse C2C12 cells)Chambers, M.A. et al. (2009) J Physiol. 587:3363. (WB: mouse muscle, C2C12)Moylan, J.S. et al. (2008) AJP Cell. Phys. 295:986. (WB: mouse C2C12)Smith, M. et al. (2007) Am J Physiol Cell Physiol 293:1947. (WB: mouse C2C12 cells)This kit contains:
KIT SUMMARY
ECM Biosciences是一家著名的生物公司，开发新的生物技术的生命科学研究。公司工程与研究机构全球发展创新的工具，以提高研究和治疗相关的神经病理学，癌症，免疫功能障碍和心血管疾病。ECM Biosciences 的科学合作重点在分子组成部分，产生细胞运动的变化，细胞形状，促进细胞运动和细胞结构确定。细胞运动的重要作用在肿瘤细胞迁移，免疫细胞浸润，以及神经和血管修复。各种蛋白质在细胞外机质（ECM）周围细胞信号蛋白在细胞内的细胞骨架，以控制细胞运动 ECM Biosciences 生物利用创新肽和抗体为基础的办法，发展新的工具来检测和操作蛋白质功能的各个方面的细胞运动，如细胞黏附，细胞迁移和细胞信号。
暂无问答
本网站将在规定时间内给予删除等相关处理。
