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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
ECM BIOSCIENCES/Anti-Rabbit Ig:DyLight® 650 Secondary Antibody/0.5 ml/RS3281
产品编号:RS3281
市  场 价:¥2500.00
场      地:美国(厂家直采)
产品分类: 抗体类>二抗>抗大鼠>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$125.00
品      牌: ECM BIOSCIENCES
公      司:ECM BIOSCIENCES
公司分类:
ECM BIOSCIENCES/Anti-Rabbit Ig:DyLight® 650 Secondary Antibody/0.5 ml/RS3281
商品介绍

DyLight® fluorophores have absorption maxima covering the entire visible light spectrum (350 nm to 777 nm), as well several key near-infrared and infrared wavelengths. Both the absorption and emission properties of the DyLight® fluorophores match the excitation and detection wavelengths of common fluorescence instrumentation used for immunocytochemistry, immunohistochemistry, and flow cytometry. The DyLight® fluorophores exhibit higher fluorescence intensity and photostability than Alexa Fluor, CyDye and LI-COR Dyes in many applications and remain highly fluorescent over a broad pH range (pH 4-9). Additionally, the water solubility of the DyLight® fluorophores allows a high dye-to-protein ratio to be achieved without causing precipitation of conjugates. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make these fluorophores attractive as fluorescent probes in a variety of fluorescence methods.

 

References
Alkhatatbeh MJ (2011) J Thromb Haemost. 9(4):844.Sarkar P. et al. (2010) J Photochem Photobiol B. 98(1):35.

Immunocytochemical labeling of Fascin phosphorylation in aldehyde fixed rabbit fibroblast. The cells were labeled with rabbit polyclonal anti-Fascin (Ser-39) phosphospecific (FP2661). The antibody was detected using goat anti-rabbit DyLight® 650.

The DyLight® 650-conjugated goat polyclonal secondary reagent reacts with the heavy chains of rabbit IgG, and the light chains of most rabbit immunoglobulins.
Goat polyclonal antibody is supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody has been affinity-purified using rabbit IgG coupled to agarose beads. Purified goat polyclonal antibody was conjugated to DyLight® 650 fluorophore (Excitation = 654 nm; Emission = 673 nm). This secondary reagent can be used to detect rabbit immunoglobulins in various immunofluorescence applications, such asimmunocytochemistry, immunohistochemistry, fluorescent western blotting, and flow cytometry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY

品牌介绍

ECM Biosciences是一家著名的生物公司,开发新的生物技术的生命科学研究。公司工程与研究机构全球发展创新的工具,以提高研究和治疗相关的神经病理学,癌症,免疫功能障碍和心血管疾病。ECM Biosciences 的科学合作重点在分子组成部分,产生细胞运动的变化,细胞形状,促进细胞运动和细胞结构确定。细胞运动的重要作用在肿瘤细胞迁移,免疫细胞浸润,以及神经和血管修复。各种蛋白质在细胞外机质(ECM)周围细胞信号蛋白在细胞内的细胞骨架,以控制细胞运动 ECM Biosciences 生物利用创新肽和抗体为基础的办法,发展新的工具来检测和操作蛋白质功能的各个方面的细胞运动,如细胞黏附,细胞迁移和细胞信号。

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