Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with β- or γ-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.
References
Wallez Y. et al. (2007) Oncogene 26:1067. Baumeister U. et al. (2005) EMBOJ 24:1686.Potter M.D. et al. (2005) J Biol. Chem. 280(36):31906.
Western blot image of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).
Immunocytochemical labeling of VE-Cadherin in aldehyde fixed andNP-40 permeabilized human NCI-H446 lung carcinoma cells. Thecells were labeled with mouse monoclonal anti-VE-Cadherin(CM0351). The antibody was detected using goat anti-mouseDyLight® 594.
*For more information, see UniProt Accession P33151
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
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KIT SUMMARY
ECM Biosciences是一家著名的生物公司，开发新的生物技术的生命科学研究。公司工程与研究机构全球发展创新的工具，以提高研究和治疗相关的神经病理学，癌症，免疫功能障碍和心血管疾病。ECM Biosciences 的科学合作重点在分子组成部分，产生细胞运动的变化，细胞形状，促进细胞运动和细胞结构确定。细胞运动的重要作用在肿瘤细胞迁移，免疫细胞浸润，以及神经和血管修复。各种蛋白质在细胞外机质（ECM）周围细胞信号蛋白在细胞内的细胞骨架，以控制细胞运动 ECM Biosciences 生物利用创新肽和抗体为基础的办法，发展新的工具来检测和操作蛋白质功能的各个方面的细胞运动，如细胞黏附，细胞迁移和细胞信号。
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