The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is widely expressed and is distributed in both the nucleus and the cytoplasm of cells. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion, and stress response. A variety of stimuli activate c-Abl kinase including integrin activation, PDGF stimulation, and binding to proteins, such as c-Jun. Tyrosine phosphorylation is important for the regulation of c-Abl kinase activity. Tyrosine 245 is located in the linker region between the SH2 and catalytic domains. Phosphorylation of Tyr-245 is involved in activation of c-Abl kinase activity. Tyrosine 412 is located in the kinase activation loop of c-Abl, and phosphorylation of this residue is required for kinase activity. Thus, phosphorylation of Tyr-245 and Tyr-412 may be critical for activation of c-Abl in a variety of cell signaling pathways.
References
Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (AP1271; lanes 3 & 4), or anti-c-Abl (Tyr-245) (AP1251; lanes 5 & 6).
Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes. Blots were probed with anti-c-Abl (lane 1), anti-c-Abl (Tyr-412) (lanes 2-5), or anti-c-Abl (Tyr-245) (lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of c-Abl (Tyr-412) (lane 4 & 8) or c-Abl (Tyr-245) (lane 3 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
Product References:
AP1271 Skora, L. et al. (2015) Eur J Haematol. doi: 10.1111 ELISA: K562, KCL-22, and Ba/F3 cell linesRS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblastsMS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western BlotRS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western BlotThis kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
AM2091 | c-Abl (C-terminal region) Mouse mAb | 50 μl | WB, E | Hu, Rt, Ms | 145 |
AP1251 | c-Abl (Tyr-245), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 145 |
AP1271 | c-Abl (Tyr-412), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 145 |
MS3001 | Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E | Ms | |
RS3251 | Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
KIT SUMMARY
The c-Abl phospho-regulation antibody sampler kit can be used to detect c-Abl activation resulting from phosphorylation of Tyr-245 and Tyr-412. The kit also includes an antibody to examine total c-Abl expression levels and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.
ECM Biosciences是一家著名的生物公司，开发新的生物技术的生命科学研究。公司工程与研究机构全球发展创新的工具，以提高研究和治疗相关的神经病理学，癌症，免疫功能障碍和心血管疾病。ECM Biosciences 的科学合作重点在分子组成部分，产生细胞运动的变化，细胞形状，促进细胞运动和细胞结构确定。细胞运动的重要作用在肿瘤细胞迁移，免疫细胞浸润，以及神经和血管修复。各种蛋白质在细胞外机质（ECM）周围细胞信号蛋白在细胞内的细胞骨架，以控制细胞运动 ECM Biosciences 生物利用创新肽和抗体为基础的办法，发展新的工具来检测和操作蛋白质功能的各个方面的细胞运动，如细胞黏附，细胞迁移和细胞信号。
暂无问答
本网站将在规定时间内给予删除等相关处理。
