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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
ECM BIOSCIENCES/Annexin Family Antibody Kit/Kit/AK6730
产品编号:AK6730
市  场 价:¥8100.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$405.00
品      牌: ECM BIOSCIENCES
公      司:ECM BIOSCIENCES
公司分类:
ECM BIOSCIENCES/Annexin Family Antibody Kit/Kit/AK6730
商品介绍
The Annexin family is composed of at least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. Annexins have roles in membrane fusion, endocytosis, secretion, and repair. Annexin A1 binds to cellular membranes in a calcium-dependent manner, promotes membrane fusion and endocytosis, and has been implicated as an anti-inflammatory mediator. Annexin A2 is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood coagulation. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.
References
Zokas, L. & Glenney, J.R. (1987) J Cell Biol. 105:2111.Feinberg, J.M. et al. (1991) J Histochem. & Cytochem. 39:955.Grewal T & Enrich C (2009) Cell Signal. 21(6):847.Boye TL & Nylandsted J (2016) Biol Chem. 397(10):961.

Western blot of human PC3 cells (lane 1), human breast tissue (lane 2), lung tissue (lane 3), skin tissue (lane 4), and brain tissue (lane 5). The blot was probed with mouse monoclonal anti-Annexin A5 antibody (AM0171) at 1:500 (lanes 1-5).

Immunocytochemical labeling of Annexin A1 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-Annexin A1 (clone M021). The antibody was detected using goat anti-mouse Ig DyLight® 594.

Mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.

Product References:

AM2981 Moylan, J. et al. (2014) Redox biology 2:910. WB: C2C12 myoblastAM2981 Gilliam, L.A. et al. (2011) AJP Lung Cell Mol Phys 300:L225. IHC: mouse diaphragm

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
AM0211
Annexin A1 Mouse mAb50 μlWB, E, IP, ICCHu38
AM0181
Annexin A1 Mouse mAb50 μlWB, E, IP, ICC, IHCHu, Rt, Ms, Bv38
AM0091
Annexin A2 Mouse mAb 50 μlWB, E, ICC, IP-MSHu36
AM2981
Annexin A2 (C-terminal region) Mouse mAb50 μlWB, E, IP, ICC, IHCHu, Rt, Ms, Ck36
AM0171
Annexin A5 Mouse mAb50 μlWB, E,IP-MSHu35
AM3301
Annexin A6 Mouse mAb50 μlWB, E, IPHu, Rt, Ms67
KIT SUMMARY

The annexin family antibody sampler kit can be used to detect the expression level of annexin A1, A2, A5, and A6. The kit includes high affinity mouse monoclonal antibodies to examine annexin A1, A2, A5, or A6 expression levels in western blot, ELISA, immunoprecipitation, and immunocytochemistry.

品牌介绍

ECM Biosciences是一家著名的生物公司,开发新的生物技术的生命科学研究。公司工程与研究机构全球发展创新的工具,以提高研究和治疗相关的神经病理学,癌症,免疫功能障碍和心血管疾病。ECM Biosciences 的科学合作重点在分子组成部分,产生细胞运动的变化,细胞形状,促进细胞运动和细胞结构确定。细胞运动的重要作用在肿瘤细胞迁移,免疫细胞浸润,以及神经和血管修复。各种蛋白质在细胞外机质(ECM)周围细胞信号蛋白在细胞内的细胞骨架,以控制细胞运动 ECM Biosciences 生物利用创新肽和抗体为基础的办法,发展新的工具来检测和操作蛋白质功能的各个方面的细胞运动,如细胞黏附,细胞迁移和细胞信号。

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