Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. Arp2 has two phosphosites, Thr-237 and Thr-238, that are evolutionarily conserved, and are phosphorylated along with Tyr-202 in response to growth factor stimulation. p41-Arc (Arpc1b) subunit Arpc1 has two isoforms in humans, Arpc1a and Arpc1b. PAK1 can bind and phosphorylate Thr-21 in Arpc1b leading to growth factor-stimulated cell motility. In addition, Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity.
References
Western blot of Arpc1b phosphorylation inA431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).
Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
Product References:
AP3871 Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 WB: mouse macrophagesAP4351 Kim, Y.B. et al. (2015) J Biol Chem. 290(34):21076 WB: PC3 cellsAP3861 Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 WB: mouse macrophagesAP4321 Ho, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. WB: human A375 melanomaAP4351 Ho, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. WB: human A375 melanomaAP3861 Spillane, M. et al. (2012) J Neurosci. 32(49):17671. ICC: Chicken embryo neuronAP3871 Kalwa, H. & Michel, T. (2011) J Biol Chem. 286(3):2320. WB: bovine aortic endothelial cellsAP3861 Kalwa, H. & Michel, T. (2011) J Biol Chem. 286(3):2320. WB: bovine aortic endothelial cellsAP3861 Spillane, M. et al. (2011) Dev Neurobiol. 71(9):747. ICC: chick dorsal root gangliaThis kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
AP3861 | Arp2 (C-terminal region) Rabbit pAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms, Ck, Fr | 44 |
AP3871 | Arp2 (Thr-237/Thr-238), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck | 44 |
AP4581 | Arp3 (C-terminal region) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck, F | 47 |
AP4321 | Arpc1b/p41-Arc (C-terminal region) Rabbit pAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms, Ck | 41 |
AP4351 | Arpc1b/p41-Arc (Thr-21), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms, Ck, F | 41 |
KIT SUMMARY
The Arp2/3 complex kit can be used to detect expression levels of Arp2, Arp3, and Arpc1b in many different species. In addition, phospho-specific antibodies are included to detect phosphorylation of Arp2 (Thr-237/Thr-238) and Arpc1b (Thr-21).
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