Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. Arp2 has two phosphosites, Thr-237 and Thr-238, that are evolutionarily conserved, and are phosphorylated along with Tyr-202 in response to growth factor stimulation. These phosphorylation events may regulate binding to the pointed end of actin filaments, and alanine substitutions of these Arp2 phosphosites inhibit membrane protrusions. Thus, phosphorylation may be another mode of Arp2/3 complex regulation in addition to the activity of nucleation-promoting factors.
References
Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with anti-Arp2 (C-terminal) (lanes 1 & 2) or anti-Arp2 (T237/T238) (lanes 3 & 4).
Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
Product References:
AP3861 Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 WB: mouse macrophagesAP3871 Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 WB: mouse macrophagesWP2601 Uenishi, E. et al. (2013) J of Bio Chem 288(36): 25851. WB: MIN6-K8 β-cellsAP3861 Spillane, M. et al. (2012) J Neurosci. 32(49):17671. ICC: Chicken embryo neuronCP2621 Williams, H.C. et al. (2012) Circ Res. 111(1):56. ICC, WB: mouse carotid artery,smooth muscleAP3861 Kalwa, H. & Michel, T. (2011) J Biol Chem. 286(3):2320. WB: bovine aortic endothelial cellsAP3861 Spillane, M. et al. (2011) Dev Neurobiol. 71(9):747. ICC: chick dorsal root gangliaAP3871 Kalwa, H. & Michel, T. (2011) J Biol Chem. 286(3):2320. WB: bovine aortic endothelial cellsWP2101 Cheney, L. et al. (2011) J Infectious Diseases. 203:1824. WB: mouse adipocytesWP2601 Cheney, L. et al. (2011) J Infectious Diseases. 203:1824. WB: mouse adipocytesWP2601 Pichot, C.S. et al. (2009) British J Cancer. 1 –10. WB: breast cancer cell linesThis kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
AP3861 | Arp2 (C-terminal region) Rabbit pAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms, Ck, Fr | 44 |
AP3871 | Arp2 (Thr-237/Thr-238), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck | 44 |
CP2581 | Coronin-1B (C-terminus) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 60 |
CP2621 | Coronin-1B (Ser-2), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 60 |
WP2101 | WASP/N-WASP Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 63 |
WP2601 | N-WASP (Tyr-256), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 65 |
KIT SUMMARY
The Arp2/3 complex regulation antibody sampler kit can be used to examine phosphorylation of the Arp2/3 complex subunit Arp2, as well as the Arp2/3 complex regulators N-WASP/WASP and Coronin-1B. The kit contains antibodies to monitor total expression levels of each of these proteins relative to site-specific phosphorylation.
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