The caspases are a group of cysteine enzymes which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.
References
Western blot analysis of Caspase expression in human Jurkat cells. The blots were probed with mouse monoclonal anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2) or mouse monoclonal anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4).
Western blot analysis of Caspase expression in human Jurkat cells. The blots were probed with mouse monoclonal anti-Caspase-7 at 1:500 (lane 1) and 1:1000 (lane 2) or mouse monoclonal anti-Caspase-8 at 1:250 (lane 3) and 1:500 (lane 4).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
Product References:
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western BlotThis kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
CM3771 | Caspase-3 (N-terminal region)Mouse mAb | 50 μl | WB, E, ICC | Hu | 32 |
CM3781 | Caspase-6 (C-terminal region) Mouse mAb | 50 μl | WB, E | Hu | 34 |
CM3811 | Caspase-7 (N-terminal region) Mouse mAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 35 |
CM3821 | Caspase-8 (C-terminal region) Mouse mAb | 50 μl | WB, E | Hu | 55 |
MS3001 | Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E | Ms |
KIT SUMMARY
The Caspase family antibody sampler kit can be used to detect the full length and cleaved products of Caspase-3, Caspase-6, Caspase-7, and Caspase-8. The kit also includes secondary reagent for detection of these antibodies.
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